扩增子多营养级基于高通量测序技术，突破传统单一微生物研究局限，实现对环境样本中细菌、真菌、古菌、真核生物等多营养级生物群落的全面解析。通过靶向扩增特定功能基因或标记基因，精准捕捉不同营养级生物的组成与丰度信息，为生态系统结构与功能研究、环境微生物群落动态监测、农业土壤健康评估等多领域提供关键数据支撑，深度挖掘样本中生物间复杂的互作关系与生态规律。
全面覆盖：同时检测多个营养级生物、一次实验即可获得环境样本中细菌、真菌、原生生物、藻类等多种生物类群的遗传信息，打破传统单一扩增子检测的局限性。
高灵敏度与准确性：采用高保真聚合酶与优化的扩增体系，结合先进的高通量测序平台，能够精准检测到低丰度物种，确保数据真实可靠，有效降低假阳性与假阴性结果。
特异性引物：针对不同营养级生物的特异性基因位点，设计多种高特异性引物，通过多轮验证确保引物扩增效率与特异性，可精准区分近缘物种，避免交叉扩增干扰。
数据分析：自主研发的生物信息学分析软件，整合多组学分析方法，从物种注释、群落结构组成、物种间互作关系、功能预测等多个维度进行深度挖掘，提供直观可视化的分析结果。
| 环境样本（使用干冰或冰袋运输） | 土壤≥5g 粪便≥2g |
| DNA样品（使用干冰或冰袋运输） | DNA总量≥500ng； 浓度≥5ng/ul； 样本数目≥30 |
发表时间 | 文章标题 | 杂志名 | 影响因子 | 合作单位 |
2024 | Hysteretic and asynchronous regime shifts of bacterial and micro-eukaryotic communities driven by nutrient loading | Water Research | 11.4 | 河海大学环境学院 |
2024 | Multitrophic interactions support belowground carbon sequestration through microbial necromass accumulation in dryland biocrusts | Soil Biology&Biochemistry | 9.8 | 中国农业大学 |
2024 | Deforestation impacts soil biodiversity and ecosystem services worldwide | PANS | 9.4 | 南京农业大学 |
2024 | Environmental heterogeneity caused by large-scale cultivation of Pyropia haitanensis shapes multi-group biodiversity distribution in coastal areas | Science of The Total Environment | 8.2 | 集美大学水产学院 |
2024 | Ecological restoration enhanced the stability of epiphytic microbial food webs of submerged macrophytes: Insights from predation characteristics of epiphytic predators | Science of the Total Environment | 8.2 | 河海大学 |
2025 | Stability characteristics of bacterial and protistan communities along an estuarine continuum: Diversity, composition and co-occurrence networks | Environmental Research | 7.7 | 河海大学环境学院 |
2024 | Body size of soil organisms is more sensitive to fertilization than their community structure: Evidence from a rice field trial | Ecological Indicators | 7 | 浙江省农业科学院环境资源与土壤肥料研究所 |
2024 | Inoculation of the Morchella importuna mycosphere with Pseudomonas chlororaphis alleviated a soil-borne disease caused by Paecilomyces penicillatus | Biology and Fertility of Soils | 5.1 | 四川省农业科学院 |
2024 | Differentiation strategies for microbial community assembly and their associations with soil functions in lemon farmland | Applied Soil Ecology | 4.8 | 中国农业科学院中国柑橘国家工程研究中心 |
2024 | Chlorine disinfectant significantly changed microfauna habitat, community structure, and colonization mode in wastewater treatment plants | Environmental Microbiology | 4.3 | 华中科技大学环境科学与工程学院 |
2024 | Mechanisms of bacterial and fungal community assembly in leaf miners during transition from natural to laboratory environments | Frontiers in Microbiology | 4 | 扬州大学植物保护学院昆虫学系 |
