微生物多样性测序三代全长扩增子宏基因组测序宏基因组Binning分析宏基因组抗性基因测序宏基因组元素循环测序高宿主污染去除宏基因组项目HiC-Meta宏基因组三代全长宏基因组宏转录组差异表达测序环境宏病毒组测序医学宏病毒组测序动植物基因组Denovo测序细菌基因组测序项目真菌基因组测序项目病毒基因组测序项目简化基因组遗传图谱简化基因组GWAS测序BSA混池测序基因组SSR开发基因组重测序真核有参转录组测序真核无参转录组测序原核链特异性转录组测序全长转录组(有参_无参)测序LncRNA测序Small RNA测序互作转录组测序比较转录组测序Meta-Barcoding(eDNA)技术研究5R 16S rRNA微生物多样性绝对定量 Spike-in种质资源数字化解决方案转座子插入测序(Tn-seq)PlantArray植物生理组平台UMI-RNA-seq技术染色体级别基因组组装Hi-C建库叶绿体、线粒体基因组测序高通量土壤生物检测Astral蛋白质组学植物单细胞核转录组动物单细胞核转录组动物单细胞转录组单菌株单细菌转录组人肠道单细菌转录组10x单细胞转录组单细胞转录组(SMART)测序靶向代谢组分析非靶向代谢组分析ATAC-seqChip-seqRip-seq全基因组甲基化分析扩增子引物列表资料下载送样指南客户文章列表
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常规三代全长引物列表

测序类型引物名称引物序列区域文献备注

细菌16S rRNA

27F5’-AGAGTTTGATCMTGGCTCAG-3’

16s V1-V9

全长1.5k

[1]Pacbio 平台

1492R

5’- CRGYTACCTTGTTACGACTT-3’

真菌ITSITS15'-CTTGGTCATTTAGAGGAAGTAA-3’

ITS 全长

700bp

[2]Pacbio 平台

ITS4

5'-TCCT CCGC TTAT TGAT ATGC-3’

偏好产甲烷古菌A1F5′-GKTTGATCCYGSCRGAG-3′16S全长1.5k[3]Pacbio 平台

1490R

5′-GGYTACCTTGTTACGACTT-3’



常规引物列表

测序类型引物名称引物序列区域文献备注
细菌16S rRNA341F

5’-CCTAYGGGRBGCASCAG-3’

16s V3-V4

[4]

PE250平台
806R

5’-GGACTACNNGGGTATCTAAT-3’

细菌16S rRNA515F

5’- GTGCCAGCMGCCGCGG-3’

16s V4-V5

[5]

PE250平台
907R

5’- CCGTCAATTCMTTTRAGTTT-3’

细菌16S rRNA515F

5’- GTGCCAGCMGCCGCGG-3’

16s V4

[6]

PE250平台
806R

5’- GGACTACHVGGGTWTCTAAT-3’

细菌16S rRNA799F

5’- AACMGGATTAGATACCCKG-3’

16s V5-V6

[7]

PE250平台
1115R

5’- AGGGTTGCGCTCGTTG-3’

真菌ITS

ITS1F

5’- CTTGGTCATTTAGAGGAAGTAA-3’

ITS1

[8]

PE250平台

ITS2R

5’- GCTGCGTTCTTCATCGATGC-3’

真核18S

TAReuk454FWD1

5’-CCAGCASCYGCGGTAATTCC-3’

18s V4

[9]

PE250平台

TAReukREV3

5’-ACTTTCGTTCTTGATYRA-3’

古菌

Arch519F

5’- CAGCCGCCGCGGTAA-3’

V4-V5

[10]

PE250平台

Arch915R

5’- GTGCTCCCCCGCCAATTCCT-3’

鱼类(海水)

MiFish_U-F

5’-GTTGGTAAATCTCGTGCCAGC-3’

eDNA

12S rRNA

[11]

PE150平台

MiFish_U-R

5’CATAGTGGGGTATCTAATCCTAGTTTG-3’

鱼类(淡水)

Tele02_F

5′-AAACTCGTGCCAGCCACC-3′

eDNA

12S rRNA

[12]

PE150平台

Tele02_R

5′-GGGTATCTAATCCCAGTTTG-3′

真核COI

MlCOIintF

5’GGWACWGGWTGAACWGTWTAYC

CYCC-3’

eDNA

[13]

PE250平台

JghHCO2198

5’-TAIACYTCIGGRTGICCRAARAAYCA-3’

产甲烷菌

Met86F

5’- GCTCAGTAACACGTGG-3’

产甲烷菌

[14]

Pacbio 平台

Met1340R

5’-CGGTGTGTGCAAGGAG-3’

固氮菌

NifH1F

5’- TGYGAYCCNAARGCNGA -3’

固氮细菌

[15]

PE250平台

NifH2R

5’- ADNGCCATCATYTCNCC-3’

丛枝真菌

AMV4.5NF

5′- AAGCTCGTAGTTGAATTTCG-3’

SSU

300bp

[16]

PE250平台

AMDGR

5′-CCCAACTATCCCTATTAATCAT-3’



非常规引物列表

测序类型引物名称引物序列区域文献备注
真核18S1380F5’-CCCTGCCHTTTGTACACAC -3’18s V9[17]PE250平台
1510R5’-CCTTCYGCAGGTTCACCTAC-3’
真核18S0817F5’-TTAGCATGGAATAATRRAATAGGA-3'18S V5-V7[18]PE250平台
1196R5'-TCTGGACCTGGTGAGTTTCC-3'
真核18SSSU_FO45’-GCTTGTCTCAAAGATTAAGCC-3’18S V1-V2[19]PE250平台
SSU_R225’-GCCTGCTGCCTTCCTTGGA-3’
古菌ArchU519F5’-CAGYMGCCRCGGKAAHACC-3’Arch 16s V4[20]PE250平台
Arch806R5’-GGACTACNSGGGTMTCTAAT-3’

氨氧化细菌

amoA-1F5'-GGGGTTTCTACTGGTGGT-3'氨氧化细菌[21]PE250平台单端分析
a moA-2R5'-CCCCTCKGSAAAGCCTTCTTC-3'

氨氧化古菌


Arch-amoAF5’-STAATGGTCTGGCTTAGACG-3’氨氧化古菌[22]PE250平台单端分析
Arch-amoAR5’-GCGGCCATCCATCTGTATGT-3’

线虫


NF15’-GCTGGTGCATGGCCCTTCTTACTT-3'线虫18S[23]PE250平台
18Sr2bR5'-TACAAAGCGCAGCGACCTAAT-3'
反硝化细菌

(nirS)

Cd3aF5’-GTSAACGTSAAGGARACSGG-3’nirS[24]PE250平台
R3cd5’-GASTTCGGRTGSGTCTTGA-3’

厌氧真菌

MN1005’-TCCTACCCTTTGTGAATTTG-3’厌氧真菌[25]PE250平台
MNGM2C5’-CTGCGTTCTTCATCGTTGCG-3’
产甲烷古菌Met86F5’- GCTCAGTAACACGTGG-3’产甲烷古菌[26]PE250平台
Met471R5’-GWRTTACCGCGGCKGCTG-3’

反硝化

细菌(nirK)

nirK583F5’- TCATGGTGCTGCCGCGKGACGG-3’nirK[27]PE250平台
nirK909R5’-GAACTTGCCGGTKGCCCAGAC-3’

反硝化细

菌(nosZ)

nosZ-F5’-CGYTGTTCMTCGACAGCCAG-3’nosZ[28]PE250平台
nosZ1622R5’-CGSACCTTSTTGCCSTYGCG-3’

反硝化细

菌(cnorB)

cnorB2F5’- GACAAGNNNTACTGGTGGT - 3’cnorB[29]PE250平台
cnorB6R5’- GAANCCCCANACNCCNGC-3’

厌氧氨氧化菌

AMX368F5’-TTCGCAATGCCCGAAAG-3’Anammox[30]PE250平台
AMX820R5’-AAAACCCCTCTACTTAGTGCCC-3’

硫酸盐还原菌

APS1F5’-TGGCAGATCATGATYMAYGG-3’aprA[31]PE250平台
APS4R5’- GCGCCAACYGGRCCRTA- 3’

藻类

Cyan359F5’-GGGGAATYTTCCGCAATGGG- 3’chloroplasts[32]PE250平台
Cyan781R5’-GACTACWGGGGTATCTAATCCCWTT- 3’

真核18S

528F5’-GCGGTAATTCCAGCTCCAA-3’18s V4 350bp[33]PE250平台
706R5’-AATCCRAGAATTTCACCTCT-3’

大型藻类

F5’-CCAGCASCYGCGGTAATTCC- 3’18S V9[34]PE150平台
R5’-CCTTCYGCAGGTTCACCTA- 3’
大型藻类Euka02-F5’-TTTGTCTGSTTAATTSCG- 3’18S V7[34]PE150平台
Euka02-R5’-CACAGACCTGTTATTGC- 3’

注明:功能引物为非常规引物,需要收合成费用;

甲方可以提供特殊引物,但是需要经过乙方的确认后收费合成。

参考文献

参考文献

副标题

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