宏基因组Binning的方法,可以在自然环境样本中有效区分每个菌株基因组信息。Binning即是从微生物群体序列中将不同个体的序列(reads或contigs)分离开的过程。主要用于微生物组的两方面应用:关联分析和单菌组装。
实验策略 | 测序量 | 项目周期 |
400bp左右小片段文库 | ≧30G raw data | 约70个工作日 |
不依赖于微生物的分离培养,环境微生物单菌基因组(框架图)研究的一种新的途径和高性价比策略;
可以得到环境中丰度较低的宏基因组,为研究低丰度微生物提供了途径;
引入了宏观生态的研究理念,对环境中微生物菌群的多样性、功能活性等宏观特征进行研究,可以更准确地反应出微生物生存的真实状态;
1对1项目服务,直接对接生信分析师,没有中间环节,沟通更高效;
多组学关联分析,可关联代谢组等研究结果,全方面深化研究。
样品类型 | 样品浓度 | 样品总量 |
取样材料或者环境样本DNA | 30ng/ul | ≧1ug
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发表时间 | 文章标题 | 杂志名 | 影响因子 | 合作单位 |
2021 | An integrated gene catalog and over 10,000 metagenome-assembled genomes from the gastrointestinal microbiome of ruminants | Microbime | 14.65 | 南京农业大学 |
2024 | Toward nitrogen recovery Cocultivation of microalgae and bacteria enhances the production of high value nitrogen rich cyanophycin | Water Research | 12.8 | 湘潭大学 |
2023 | Iron oxides act as an alternative electron acceptor for aerobic methanotrophs in anoxic lake sediments | Water Research | 12.8 | 中国科学院 南京地理与湖沼学研究所 |
2023 | From waste to resource: Metagenomics uncovers the molecular ecological resources for plastic degradation in estuaries of South China | Water Research | 12.8 | 辽宁省海洋与渔业 科学研究院 |
2024 | Distinct microbial hydrogen and reductant disposal pathways explain interbreed variations in ruminant methane yield | The ISME Journal | 11.217 | 中国科学院 农业研究所 |
2024 | Soil redox status governs within-field spatial variation in microbial arsenic methylation and rice straighthead disease | The ISME Journal | 11 | 南京农业大学 |
2023 | Methylotrophic methanogens and bacteria synergisticallydemethylate dimethylarsenate in paddy soil and alleviate ricestraighthead disease
| The ISME Journal | 11 | 南京农业大学 |
2024 | Tracking investigation of archaeal composition and methanogenesis function from parental to offspring pigs
| Science of the Total Environment | 9.8 | 浙江省农产品安全与营养研究所 |
2024 | From disinfection to pathogenicity: Occurrence, resistome risks and assembly mechanism of biocide and metal resistance genes in hospital wastewaters
| Environ Pollut | 8.9 | 上海交通大学医学院附属瑞金医院感染控制科 |
2024 | Characterization and genetic determination of a newly isolated cotinine-degrading bacterium Terrabacter sp. strain cot-2 from synergistic consortium
| Journal of Cleaner Production | 8.3 | 南京农业大学 |
2023 | Straw-based compost cultivation disproportionally contributes to the environmental persistence of antibiotic resistance from raw cattle manure to organic vegetables | Microbiological Research | 6.7 | 南京农业大学 |