病毒(Virus)由一种核酸分子(DNA或RNA)与蛋白质(Protein)构成或仅由蛋白质构成(如 朊病毒)。根据遗传物质的组成,将所有已知的病毒分为DNA病毒——单链DNA病毒,DNA病毒——双链DNA病毒,DNA与RNA反转录病毒,RNA病毒——双链RNA病毒,RNA病毒——单链RNA病毒、裸露RNA病毒及类病毒等八大类群。此外,还增设亚病毒因子一类。目前病毒约4000种,设有三个病毒目,64个病毒科,9个病毒亚科,233个病毒属,其中29个病毒属为独立病毒属。亚病毒因子类群,不设科和属。包括卫星病毒和prion(传染性蛋白质颗粒或朊病毒)。一些属性不很明确的属称暂定病毒属。
病毒根据宿主类型的不同,可以分为噬菌体(细菌病毒)、植物病毒(烟草花叶病毒)和动物病毒(如禽流感病毒、天花病毒和HIV等)。
病毒基因组结构简单,一般只含有单一核酸,在探究病毒的毒力系统、病毒的基因组进化与演变历程方面意义深远,可以为揭示病毒流行病变化趋势等提供有力参考。
病毒全基因组测序,是指基于第二代高通量测序技术,对病毒全基因组进行测序,利用生物信息分析手段,得到病毒的全基因组序列,解析编码信息,并获得相应的变异信息。
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分析病毒感染样本血清,-80 ℃保存,干冰运输; | DNA/cDNA总量≧2ug,浓度≧100ng/ul,无降解,无粘稠,无颜色异常,无RNA/蛋白质污染; 干冰寄送,使用无DNase管存放,标记清晰。 |
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- 对基因组已知的病毒进行基因组重测序,进行多态性分析;
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- 基因预测、功能注释和比较基因组学(SNP、InDel. Pan-genome)分析;
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发表时间 | 文章标题 | 杂志名 | 影响因子 | 合作单位 |
2023 | Biological and Genomic Characteristics of MaMV-DH01, a Novel Freshwater Myoviridae Cyanophage Strain | Microbiology Spectrum | 9.043 | 河海大学 |
2024 | Physiological and metabolic insights into the first cultured anaerobic representative of deep-sea Planctomycetes bacteria | eLife | 8.713 | 中国科学院海洋研究所深海研究中心 |
2021 | Isolation, characterization and comparison of lytic Epseptimavirus phages targeting Salmonella | Food Research International | 6.475 | 大连理工大学 |
2022 | Isolation and characterization of a novel phage vB_ValP_VA-RY-3 infecting Vibrio alginolyticus | Virus Research | 6.286 | 大连理工大学 |
2022 | RetS Regulates Phage Infection in Pseudomonas aeruginosa via Modulating the GacS/GacA Two-Component System | Journal of Virology | 5.942 | 中国海洋大学 |
2021 | A Novel Polyvalent Bacteriophage vB_EcoM_swi3 Infects Pathogenic Escherichia coli and Salmonella enteritidis | Front Microbiol | 5.64 | 青岛农业大学 |
2020 | The endolysin of the Acinetobacter baumannii phage vB_AbaP_D2 shows broad antibacterial activity | Microbial Biotechnology | 5.328 | 大连理工大学 |
2022 | Biological and genomic characteristics of two bacteriophages isolated from sewage, using one multidrug-resistant and one non-multidrug-resistant strain of Klebsiella pneumoniae | Frontiers in Microbiology | 5.2 | 中国农业大学 |
2020 | A newly recorded Rickettsia of the Torix group is a recent intruder and an endosymbiont in the whitefly Bemisia tabaci | Environmental Microbiology | 5.147 | 浙江大学 |
2022 | Isolation and Characterization of a Novel Vibrio natriegens—Infecting Phage and Its Potential Therapeutic Application in Abalone Aquaculture | Biology | 5.079 | 厦门大学 |
2021 | Isolation and Identifification of Type F Bovine Enterovirus from Clinical Cattle with Diarrhoea | Viruses | 5.048 | 南京农业大学 |
2022 | Genetic and Pathogenic Characterization of a New Iridovirus Isolated from Cage-Cultured Large Yellow Croaker (Larimichthys crocea) in China | Viruses | 5.048 | 浙江海洋大学,福建农林大学 |
2022 | Genomic and phylogenetic analysis of the first myovirus isolated from Oceanospirillaceae, representing a novel viral cluster prevalent in polar oceans | Applied and Environmental Microbiology | 5.005 | 中国海洋大学 |
2024 | Isolation and characterization of a novel temperate bacteriophage infecting Aeromonas hydrophila isolated from a Macrobrachium rosenbergii larvae pond | Virus Res | 5 | 浙江师范大学 |
2024 | Vibrio cyclitrophicus phage encoding gene transfer agent fragment, representing a novel viral family | VIROLOGY | 5 | 中国海洋大学 |