微生物多样性测序三代全长扩增子宏基因组测序宏基因组Binning分析宏基因组抗性基因测序宏基因组元素循环测序高宿主污染去除宏基因组项目HiC-Meta宏基因组三代全长宏基因组宏转录组差异表达测序环境宏病毒组测序医学宏病毒组测序动植物基因组Denovo测序细菌基因组测序项目真菌基因组测序项目病毒基因组测序项目简化基因组遗传图谱简化基因组GWAS测序BSA混池测序基因组SSR开发基因组重测序真核有参转录组测序真核无参转录组测序原核链特异性转录组测序全长转录组(有参_无参)测序LncRNA测序Small RNA测序互作转录组测序比较转录组测序Meta-Barcoding(eDNA)技术研究5R 16S rRNA微生物多样性绝对定量 Spike-in种质资源数字化解决方案转座子插入测序(Tn-seq)PlantArray植物生理组平台UMI-RNA-seq技术染色体级别基因组组装Hi-C建库叶绿体、线粒体基因组测序高通量土壤生物检测Astral蛋白质组学植物单细胞核转录组动物单细胞核转录组动物单细胞转录组单菌株单细菌转录组人肠道单细菌转录组10x单细胞转录组单细胞转录组(SMART)测序靶向代谢组分析非靶向代谢组分析ATAC-seqChip-seqRip-seq全基因组甲基化分析扩增子引物列表资料下载送样指南客户文章列表
021-31021022 ;13032137192


产品介绍

转录组测序的研究对象为特定细胞在某一功能状态下所能转录出来的所有mRNA。转录组测序能够从整体水平研究基因表达量以及基因结构,揭示特定生物学过程中的分子机理;目前已广泛应用于基础研究、临床诊断、药物研发和分子育种等领域。

真核有参转录组测序,是针对已有参考基因组的真核生物特定组织或细胞在某一状态下转录出来的RNA进行建库测序通过与参考基因组比对,既可快速分析mRNA序列和丰度信息,又可对基因结构和产生的新转录本进行分析;识别可变剪切位点,以及不同转录本之间的单碱基差异等。针对人、小鼠等模式生物,还可进行蛋白互作网络分析,深入研究基因功能,指导网络调控机制的进一步研究。


技术流程

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送样要求


RNA总量

≥2ug

RNA浓度

≥100 ng/ul

RIN

≥7.0




技术优势


  • 识别可变剪切位点,以及不同转录本之间的单碱基差异;

  • 无需转录组拼接,即可对基因结构和产生的新转录本进行分析;
  • 可对低起始量或完整度低的样品进行文库构建;

  • 11项目服务,直接对接生信分析师,没有中间环节,沟通更高效
  • 多种个性化分析方案,全方位满足研究需要,助力科研成功。



分析示例


真核有参转录组测序.png



部分凌恩客户文章


发表时间文章标题杂志名影响因子合作单位
2024Early‑life ruminal microbiome‑derived indole‑3‑carboxaldehyde and prostaglandin D2 are efective promoters of rumen developmentGenome Biology17.906南京农业大学
2022Long-term efect of epigenetic modifcation in plant–microbe interactions: modifcation of DNA methylation induced by plant growth promoting bacteria mediates promotion processMicrobiome16.837南京农业大学
2022Toxicity Evaluation of Decabromodiphenyl Ethane (Dbdpe) to Pleurotus Ostreatus for Improving its Biodegradation: Oxidative Stress, Morphology and TranscriptomicsJournal of Hazardous Materials14.224

哈尔滨

工业大学

2022Transcriptome analysis reveals the molecular mechanisms of Phragmites australis tolerance to CuO-nanoparticles and/or flood stress induced by arbuscular mycorrhizal fungiJournal of Hazardous Materials14.224武汉理工大学
2023Comparative toxicogenomics of benzotriazole ultraviolet stabilizers at environmental concentrations in Asian clam (Corbicula fluminea): Insight into molecular networks and behaviorJ Hazard Mater13.6内蒙古大学
2023Engineered Bio-Heterojunction with Infection-Primed H 2 S Liberation for Boosted Angiogenesis and Infectious Cutaneous RegenerationSmall13.3四川大学
2024MXene-Decorated Nanofibrous Membrane with Programmed Antibacterial and Anti-Inflammatory Effects via Steering NF-κB Pathway for Infectious Cutaneous RegenerationSmall13.3四川大学
2023MXene-Decorated Nanofibrous Membrane withProgrammed Antibacterial and Anti-Inflammatory Effects viaSteering NF-B Pathway for Infectious CutaneousRegenerationSmall13.3四川大学
2022Cell-subpopulation alteration and FGF7 activation regulate the function of tendon stem/progenitor cells in 3D microenvironment revealed by single-cell analysisBiomaterials12.479

浙江大学

医学院

2023Humic acid-dependent respiratory growth of Methanosarcina
acetivorans involves pyrroloquinoline quinone
The ISME Journal   11山东大学
2022Tumour cells are sensitised to ferroptosis viaRB1CC1-mediated transcriptional reprogrammingclinical and translationnal medicine10.892同济大学医学院附属上海第十人民医院
2021Selenium nanoparticles ameliorate Brassica napus L. cadmium toxicity by inhibiting the respiratory burst and scavenging reactive oxygen speciesJournal of Hazardous Materials10.588山东大学
2021Environmentally relevant concentrations of sertraline disrupts behavior and the brain and liver transcriptome of juvenile yellow catfsh (Tachysurus fulvidraco): Implications for the feeding and growth axisJournal of Hazardous Materials10.588中国科学院南京地理与湖泊研究所
2021In-cytoplasm mitochondrial transplantation for mesenchymal stem cells engineering and tissue regenerationBioengineering&translational medicine10.299浙江大学&浙江大学医学院
2021Maintaining manganese in tumor to activate cGAS-STING pathway evokes a robust abscopal anti-tumor effectJournal of Controlled Release9.776

南京大学

医学院

2023Undernutrition Disrupts Cecal Microbiota and Epithelium Interactions, Epithelial Metabolism, and Immune Responses in a Pregnant Sheep ModelMicrobiology Spectrum9.043安徽农业大学
2023Using comparative transcriptome analysis to identify molecular response mechanisms to salinity stress in channel catfish (Ictalurus punctatus)Environmental Pollution8.9江苏省淡水水产研究所
2023Short C-terminal Musashi-1 proteins regulate pluripotency states in embryonic stem cellsCell Reports8.8同济大学
2023Adverse effects of polystyrene nanoplastics on sea cucumber Apostichopus japonicus and their association with gut microbiota dysbiosisChemosphere8.8辽宁省海洋与渔业科学研究院
2024Integrative transcriptomic and metabolomic analyses reveals the toxicity and mechanistic insights of bioformulated chitosan nanoparticles against Magnaporthe oryzae
Chemosphere8.8浙江省作物病原虫生物学重点实验室
2024Wolbachia symbionts control sex in a parasitoid wasp using a horizontally acquired geneCurrent Biology8.1沈阳农业大学
2023Soybean type-B response regulator GmRR1 mediates phosphorus uptake and yield by modifying root architecturePlant Physiol8.005南京农业大学
2019Ferroptosis is governed by differential regulation of transcription in liver cancerRedox Biology7.793上海同济大学第十人民医院
2020The Wnt/β-catenin/VASP positive feedback loop drives cell proliferation and migration in breast cancerOncogene7.791武汉大学
2020Gut microbiota regulate tumor metastasis via circRNA/miRNA networksGut Microbes7.74

同济大学

医学院

2022Reductions in bacterial viability stimulate the production of Extra-intestinal Pathogenic Escherichia coli (ExPEC) cytoplasm-carrying Extracellular Vesicles (EVs)PLOS Pathogens7.464南京农业大学
2023Comparing the inhibitory effects of CuO-rGO, CuO NPs, and CuCl2 on the oomycete Phytophthora sojae: Insights from phenotypic and transcriptomic analysesEnvironmental science. Nano7.3西北农林科技大学
2023Discovery of a Novel, Potent, Orally Active, and Safe Inhibitor Targeting Human Mitochondrial RNA PolymeraseJournal of Medicinal Chemistry7.3中国药科大学
2023Cadmium induced immunosuppression through TLR-IκBα-NFκB signaling by promoting autophagic degradationEcotoxicol Environ Saf6.8江苏大学第四附属医院
2024Integrated analysis of physiological, transcriptome, and metabolome analyses of the gills in Solenaia oleivora under ammonia exposureEcotoxicology and Environmental Safety6.8南京农业大学无锡渔业学院
2024Transcriptomic and metabolomic analyses reveal that lignin biosynthesis contributes to bayberry (Myrica rubra) defence responses against twig blightPlant Stress6.8浙江大学
2022An ARF1-binding factor triggering programmed cell death and periderm development in pear russet fruit skinHorticulture Research6.793浙江省农业科学院
2022Understanding water conservation vs. profligation traits in vegetable legumes through a physio-transcriptomic-functional approachHorticulture Research6.793中国计量大学
2022Reactive Oxygen Species Partly Mediate DNA Methylation in Responses to Different Heavy Metals in PokeweedFrontiers in Plant Science6.627南京农业大学
2022Functional physiological phenotyping and transcriptome analysis provide new insight into strawberry growth and water consumption
Frontiers in Plant Science6.627

山东省果树

研究所

2022Alternative Splicing Dynamics During the Lifecycle of Salvia miltiorrhiza Root Revealed the Fine Tuning in Root Development and Ingredients BiosynthesisFrontiers in Plant Science6.627第二军医大学
2022Pentatricopeptide Repeat Gene-Mediated Mitochondrial RNA Editing Impacts on Rice Drought ToleranceFrontiers in Plant Science6.627华中农业大学
2022Determination of the Effects of Duodenal Infusion Soy Protein Hydrolysate on Hepatic Glucose and Lipid Metabolism in Pigs Through Multi-Omics AnalysisFrontiers in Nutrition6.59南京农业大学
2022Multi-Omics Landscape of DNA Methylation Regulates Browning in “Fuji” AppleFrontiers in Nutrition6.59

中国农业

科学院

2021Feeding Frequency Modulates the Intestinal Transcriptome Without Affecting the Gut Microbiota in Pigs With the Same Daily Feed IntakeFrontiers in Nutrition6.576南京农业大学
2023Volatile secondary metabolome and transcriptome analysis reveals distinct regulation mechanism of aroma biosynthesis in Syringa oblata and S. vulgarisPlant Physiol Biochem6.5山东农业大学
2022Transcriptome profiling reveals toxicity mechanisms following sertraline exposure in the brain of juvenile zebrafish (Danio rerio)Ecotoxicology and Environmental Safety6.291中国科学院南京地理与湖沼学研究所
2021Urine stem cells are equipped to provide B cell survival signalsStem Cells6.277

南澳大利亚

大学

2022The strigolactone receptor SlDWARF14 plays a role in photosynthetic pigment accumulation and photosynthesis in tomatoPlant Cell Rep6.2南京农业大学
2023Bacteroides-derived isovaleric acid enhances mucosal immunity by facilitating intestinal IgA response in broilersJournal of Animal Science and Biotechnology6.175华中农业大学
2022Duodenal infusion of soy protein hydrolysate activates cAMP signaling and hypothalamic neurotransmitter synthesis in pigsFood Funct6.1南京农业大学
2022Heat Stress Weakens the Skin Barrier Function in Sturgeon by Decreasing Mucus Secretion and Disrupting the Mucosal Microbiota.Frontiers in Microbiology6.064四川农业大学
2023Alteration of gastric microbiota and transcriptome in a rat with gastric intestinal metaplasia induced by deoxycholic acidFrontiers in Microbiology6.064

南京中医药

大学