QTL定位——数量性状在遗传上是由很多基因控制的,很大程度上受环境因素影响,控制数量性状的主效基因位点称为QTL(Quantitative Trait Locus)。QTL定位就是确定数量性状基因在染色体上的位置的方法。
微卫星标记(Microsatellite)也称为SSR,是广泛分布在真核生物基因组中的简单重复序列。使用全基因组低深度测序,可以在基因组序列中迅速鉴定SSR序列,并进行批量引物设计,用于SSR标记开发。
单核苷酸多态性(SNP),是基因组水平上由单个核苷酸变异所引起的DNA序列多态性,广泛存在于基因组种,可作为重要的分子标记应用于医学及农业研究。
简化基因组测序技术利用限制性核酸内切酶识别基因组相关位点并进行酶切反应,然后对酶切获得的Tag序列进行高通量测序,可快速开发出成千上万的SNP,SSR分子标记,用于分子标记开发、群体遗传分析、遗传图谱构建、QTL定位、全基因组关联分析、群体研究与分子育种领域等等。
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- 团队人员稳定,项目经验丰富,核心技术负责人均10年以上从业经验,经验丰富创新性强,可提供个性化分析服务;
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样品类型 | 样品材料或者DNA |
DNA总量 | ≧2ug |
DNA浓度 | ≧30ng/ul |
发表时间 | 文章标题 | 杂志名 | 影响因子 | 合作单位 |
2019 | Bi-directional selection in upland rice leads to its adaptive differentiation from lowland rice in drought resistance and productivity. | Molecular Plant | 9.326 | 上海农业大学 |
2023 | Construction of a high-density genetic map for yardlong bean and identification of ANT1 as a regulator of anthocyanin biosynthesis | Horticulture Research | 8.7 | 江苏省农业科学院 |
2023 | Large-scale population structure and genetic architecture of agronomic traits of garlic | Horticulture research | 8.7 | 中国农业科学院蔬菜花卉研究所 |
2016 | Genomic regions, cellular components and gene regulatory basis underlying pod length variations in cowpea (V. unguiculata L. Walp) | Plant Biotechnology journal | 6.305 | 浙江农学院 |
2022 | Genome-wide SNPs reveal the fine-scale population structure of Laodelphax striatellus in China using double-digest restriction site-associated DNA sequencing | Genomics | 6.2 | 沈阳农业大学 |
2017 | Genome resources for climate-resilient cowpea, an essential crop for food security | the Plant journal | 5.901 | 浙江农学院 |
2017 | The genetic architecture of shoot–root covariation during seedling emergence of a desert tree, Populus euphratica | the Plant journal | 5.901 | 北京林业大学 |
2021 | Identifification and Validation of a Core Single-Nucleotide Polymorphism Marker Set for Genetic Diversity Assessment,Fingerprinting Identifification, and Core Collection Development in Bottle Gourd | Frontiers in Plant Science | 5.754 | 浙江省农科院蔬菜研究所 |
2022 | Mapping Floral Genetic Architecture in Prunus mume, an Ornamental Woody Plant | Frontiers in Plant Science | 5.753 | 北京林业大学 |
2019 | A cation diffusion facilitator,GmCDF1,negatively regulates salt tolerance in soybean | PLOS genetics | 5.54 | 南京农业大学 |
2021 | Functional physiological phenotyping with functional mapping: A general framework to bridge the phenotype-genotype gap in plant physiology | iScience | 5.458 | 武汉大学 |
2023 | Genomic evidence reveals high genetic diversity in a narrowly distributed species and natural hybridization risk with a widespread species in the genus Geodorum | BMC Plant Biology | 5.3 | 广西壮族自治区、中国科学院广西植物研究所 |