微生物多样性测序三代全长扩增子5R 16S rRNA微生物多样性绝对定量 Spike-in宏基因组测序三代宏基因组宏基因组Binning分析宏基因组抗性基因分析宏基因组元素循环分析高宿主污染去除宏基因组项目宏转录组差异表达测序环境宏病毒组测序医学宏病毒组测序高通量16S rDNA菌种鉴定三代DNA宏病毒组MIG-seq肠道宏基因组绝对定量多营养级扩增子扩增子双因素分析动植物基因组Denovo测序细菌基因组测序项目真菌基因组测序项目病毒基因组测序项目简化基因组遗传图谱eQTL简化基因组GWAS测序BSA混池测序基因组SSR开发基因组重测序真核有参转录组测序真核无参转录组测序原核链特异性转录组测序全长转录组(有参_无参)测序LncRNA测序Small RNA测序mGWASMeta-Barcoding(eDNA)技术研究种质资源数字化解决方案转座子插入测序(Tn-seq)PlantArray植物生理组平台染色体级别基因组组装Hi-C建库叶绿体、线粒体基因组测序定制生信分析服务器数据库开发构建服务高通量土壤生物检测Astral蛋白质组学植物单细胞核转录组动物单细胞核转录组动物单细胞转录组单菌株单细菌转录组人肠道单细菌转录组单细胞转录组(SMART)测序靶向代谢组分析非靶向代谢组分析ATAC-seqChip-seqRip-seq全基因组甲基化分析扩增子引物列表资料下载送样指南客户文章列表
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产品介绍

非靶向性代谢组学Untargeted Metabolomics又称为发现代谢组学,在有限的相关研究和背景知识的基础上,采用LC-MSGC-MSNMR技术,无偏向性的检测细胞、组织、器官或 者生物体内受到刺激或扰动前后所有小分子代谢物(主要是相对分子量1000 Da以内的内源性小分子化合物)的动态变化,并通过生信分析筛选差异代谢物,对差异代谢物进行通路分析,揭示其变化的生理机制。目前,非靶向代谢组学分析在生物标志物的发现、疾病的诊断和机制研究等方面应用广泛,并且为解决一些疾病机制研究的瓶颈问题提供了新的思路和方向。


技术流程


图片2.jpg


适用范围

1、生物样本中复杂代谢产物的检测

2、寻找疾病的生物标志物

3、作用代谢通路机制



送样要求(适用于全代谢组分析)


项目类型

样品类型

推荐送样量

LC-MS

&

GC/MS

动物组织

200mg

粪便

300mg

尿液

250ul

培养细胞

107

唾液

1ml

植物叶片

2000mg

微生物

120mg菌体

土壤

10g干重

血液

300ul


  • 样本过液氮速冻,-80℃保存,干冰运输。

  • 特殊样本详询技术支持
  • 建议冻存管、1.5ml-2ml离心管保存样本,植物样品可用取样袋,不建议使用异型管。


分析示例


非靶向代谢组分析.jpg


部分凌恩客户文章

发表时间

文章标题

杂志名

影响因子

合作单位

2024

Purines enrich root-associated Pseudomonas and improve wild soybean growth under salt stress

Nature Communications

16.6

中国农业科学院

2023

Impact of deoxynivalenol on rumen function, production, and health of dairy cows: Insights from metabolomics and microbiota analysis

J Hazard Mater

13.6

吉林农业大学

2024

Sustainable lipids production in algal-bacterial consortia under low light intensity: Regulation of light–dark on fatty acid composition

Chemical Engineering Journal

13.4

湘潭大学

2024

Vermicomposting preferably alters fungal communities in wasted activated sludge and promotes the production of plant growth-promoting biostimulants in the vermicompost

Chemical Engineering Journal

13.3

中国地质大学

2024

Bio-sorption capacity of cadmium and zinc by Pseudomonas monteilii with heavy-metal resistance isolated from the compost of pig manure

Bioresource Technology

11.4

西北农林大学

2024

Human microbiota from drug-naive patients with obsessive-compulsive disorder drives behavioral symptoms and neuroinflammation via succinic acid in mice

Molecular Psychiatry

11

上海交通大学

2023

Degradation and potential metabolism pathway of polystyrene by bacteria from landfill site

Environmental Pollution

8.9

北京科技大学

2024

Fatty acid modification of casein bioactive peptides nano-assemblies, synthesis, characterization and anticarcinogenic effect

Int J Biol Macromol

8.2

青岛大学

2023

Dynamic Analysis of Metabolomics Reveals the Potential Associations between Colonic Peptides and Serum Appetite-related Hormones

Food Research International

8.1

南京农业大学

2024

Untargeted metabolomics and PacBio analysis on bioactive components and microbial community in co-fermentation of black soldier fly larva

Food Research International

7

热带农业科学院

2024

Integrated analysis of physiological, transcriptome, and metabolome analyses of the gills in Solenaia oleivora under ammonia exposure

Ecotoxicol Environ Saf

6.8

南京农业大学

2024

Chronic lead poisoning–induced budgerigar liver damage, gut microbiota dysbiosis, and metabolic disorder

Ecotoxicology and Environmental Safety

6.8

安徽医科大学

2024

Transcriptomic and metabolomic analyses reveal that lignin biosynthesis contributes to bayberry (Myrica rubra) defence responses against twig blight

Plant Stress

6.8

浙江大学

2025

Discovery of a Highly Potent and Selective Tyrosine Kinase 2 (TYK2) Degrader with In Vivo Therapeutic Efficacy in a Murine Psoriasis Model

Journal of Medicinal Chemistry

6.8

中国药科大学

2024

Huangqi Jianzhong decoction improves gastric intestinal metaplasia in rats by regulating the gut‒thyroid axis

Phytomedicine

6.7

南京中医药大学

2024

Efects of diferent energy levels in low-protein diet on liver lipid metabolism in the late-phase laying hens through the gut-liver axis

Journal of Animal Science and Biotechnology

6.3

云南省农业大学

2023

Heptamethoxyflavone Alleviates Metabolic Syndrome in High-Fat Diet-Fed Mice by Regulating the Composition, Function, and Metabolism of Gut Microbiota

Journal of Agricultural and Food Chemistry

6.1

美国新泽西州罗格斯大学

2023

The combination of metagenome and metabolome to compare the differential effects and mechanisms of fructose and sucrose on the metabolic disorders and gut microbiota in vitro and in vivo

Food & Function

6.1

浙江省农业科学院

2024

Host Metabolic Alterations Mediate Phyllosphere Microbes Defense upon Xanthomonas oryzae pv oryzae Infection

Journal of Agricultural and Food Chemistry

6.1

浙江大学生物研究所

2024

PacBio sequencing combined GC–IMS approach to investigate the flavor formation of Jinhua ham prepared using Taihu black pigs

LWT

6

浙江省农业科学院