DNA甲基化是重要的表观遗传学标记信息，在维持正常细胞功能、遗传印记、胚胎发育及人类肿瘤发生中起着重要作用。获得全基因组范围内所有甲基化位点及水平数据，对表观遗传学的时空特异性研究具有重要意义。全基因组甲基化测序（Whole Genome Bisulfite Sequencing, WGBS）技术,采用亚硫酸氢盐处理基因组DNA，使未发生甲基化的胞嘧啶（C）被转化为尿嘧啶（U），而甲基化的胞嘧啶不变。亚硫酸氢盐处理后的DNA进行全基因组测序，并与参考基因组进行比对，从基因组水平实现单碱基分辨率的、高精确度甲基化水平分析，广泛应用于细胞分化、组织发育、动植物育种及人类健康与疾病研究等领域。
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- 检测范围广，能够对全基因组范围内的甲基化区域进行研究；
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- 可靠性高：直接对甲基化片段进行测序定量，无交叉反应和背景噪音
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基因表达调控、肿瘤研究、染色质重塑、遗传印记、疾病研究、胚胎发育、环境与表观遗传、表观异质性等等。 |
发表时间
| 文章标题
| 杂志名
| 影响因子
| 合作单位
|
2022
| Long-term efect of epigenetic modifcation in plant–microbe interactions: modifcation of DNA methylation induced by plant growth-promoting bacteria mediates promotion process
| Microbiome
| 14.65 | 南京农业大学 生命科学学院 |
| 2022 | Multi-Omics Landscape of DNA Methylation Regulates Browning in “Fuji” Apple
| Frontiers in Nutrition
| 6.576
| 中国农业科学院
|
| 2022 | DNA methylation reprogramming provides insights into light-induced anthocyanin biosynthesis in red pear
| Plant Science | 5.363
| 南京农业大学
|
| 2021 | Genome-Wide DNA Methylation Analysis of Soybean Curled-Cotyledons Mutant and Functional Evaluation of a Homeodomain-Leucine Zipper (HD-Zip) I Gene GmHDZ20
| Frontiers in plant science
| 5.753
| 南京农业大学
|
| 2020 | Genome-Wide DNA Methylation and RNA Analysis Reveal Potential Mechanism of Resistance to Streptococcus agalactiae in GIFT Strain of Nile Tilapia (Oreochromis niloticus)
| Journal of Immunology
| 4.886
| 中国水产科学研究院 |
| 2019 | DNA Methylation Analysis of the Citrullus lanatus Response to Cucumber Green Mottle Mosaic Virus Infection by Whole-Genome Bisulfite Sequencing
| genes
| 3.331
| 浙江省农业科学院
|
| 2017 | Transgenerational epimutations induced by multi-generation drought imposition mediate rice plant’s adaptation to drought condition | Scientific Reports
| 4.259
| 华中农业大学
|
| 2016 | Methylation interactions in Arabidopsis hybrids require RNA-directed DNA methylation and are influenced by genetic variation
| PNAS
| 9.423
| 中国科学院 |
2015
| An ABA-mimicking ligand that reduces water loss and promotes drought resistance in plants
| Cell Research
| 15.939
| 中国科学院 |
2015
| Regulation of Active DNA Demethylation by a Methyl-CpG-Binding Domain Protein in Arabidopsis thaliana
| PLOS Genetics
| 5.54
| 北京大学 |
