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产品介绍

宏基因组(metagenome是指特定环境中全部生物(微生物)遗传物质的总和。宏基因组测序以特定环境中的整个微生物群落作为研究的对象,不需对微生物进行分离培养,而是提取环境微生物总DNA进行研究。其摆脱了传统研究中微生物分离培养的技术限制,在基因组水平解读微生物群体的多样性和丰度,探索微生物与环境及宿主之间的关系。目前,第二代高通量测序技术在宏基因组的研究上已被广泛应用。第二代高通量测序平台具有通量高、准确性高、速度快、信息全等特点,加快了宏基因组测序在鉴定低丰度的微生物群落,挖掘更多基因资源方面的应用。


分析流程

宏基因组流程.png



技术参数


实验策略

测序量

项目周期

400bp左右小片段文库

常规 10G-20G raw data

50个工作日



技术特色

  • 无需分离培养微生物,突破传统纯培养技术限制,为研究和开发超 99% 的未可培养微生物提供了新途径与有效策略;

  • 基于三十余种数据库进行功能注释,解析微生物基因资源,为多领域挖掘生物分子机制提供助力;

  • 可以得到环境中丰度较低的微生物,为研究低丰度微生物提供了途径;

  • 分析内容涵盖六大分析模块,80+分析内容,结果图可直接用于文章发表;

  • 多组学关联分析,可关联代谢组等研究结果,全方面深化研究。


送样要求


样品类型

送样要求

保存及运输

常规土壤

采样时应去除地表杂质,根据需要挖取相应深度(如 5~20 cm)的土壤,置于冰上运至实验室。去除可见杂质,建议过2 mm 无菌筛网。同一样方多点样本等量混合均匀后取 5~10 g,保存无菌 EP 管中。

样本-80℃或液氮中长期保存,干冰运输
根际土壤

矮小植物:收集植物植株,去除根部大块土壤;摇动植株去除松散土壤,使用无菌刷子收集根部附着紧密的土壤;同一样方多点样本等量混合均匀,过2mm筛后,分装至2mL或更大体积的EP管或冻存管中;每管土壤含量大概0.25~0.5g,需保证送样量在1~2g

高大林木:采集地面下10-20cm根际土壤,置于冰上运至实验室。去除可见杂质,建议过2-5 mm 无菌筛网。同一样方多点样本等量混合均匀后取 5~10 g,保存无菌 EP 管中。

水体

采样后进行滤膜过滤,选择合适孔径的滤膜,对于澄清水体或者略浑浊水体,选用0.22μm 或者 0.45μm 的滤膜,取样体积大于 5L;对于浑浊水体,建议先用3-4cm 滤膜过滤一遍,再用小孔径的滤膜过滤;送样量:滤膜 1-2 张;水体泥样的采集提供大于 5g 样本。

活性污泥
地表沉积物

活性污泥:从活性污泥装置中取大于 10 ml 的悬浮污泥样本(约 5-10 g 沉降物),保存无菌 EP 管中,放入液氮或置于冰上,立即运至实验室。若液态污泥样本沉降物较少,可适当增加取样量。

水体/地表沉积物:取距离水底/地表 0-10 cm(具体按实验需要而定)的沉积物 5~10 g,保存无菌取样袋或EP 管中,置于冰上运送至实验室。

空气

使用空气抽滤机,使空气通过 0.22 μm 滤膜,滤膜上有可见覆盖物(过滤时间越长,收集的空气中的灰尘越多,菌数量越多),收集完成后取下滤膜。滤膜置于冻存管中,液氮速冻,干冰寄送到实验室。

粪便

无菌牙签或用无菌勺挖取粪便中段没有接触空气和地面的部分,挖取一满勺(3-5 g)。小鼠至少 3-5 粒粪便。将已取的粪便样品分装至2mL EP管(无菌)或冻存管(无菌)中,每管粪便量为0.5~2g,每个样品分装2~3管备份。

肠道内容物

在实验对象死亡后,无菌条件下,取出整个肠道,用无菌解剖刀切取所需肠段的,用无菌手术刀挖取内容物 ,立即放在冰上进行分装并标记,分装至2mL EP管(无菌)或冻存管(无菌)中,每管组织量为0.5~2g,每个样品分装2~3管备份



分析示例

宏基因组测序.png



部分凌恩客户文章


发表时间

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12.2

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Stereoselective behavior of naproxen chiral enantiomers in promoting horizontal transfer of antibiotic resistance genes

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Early-life ruminal microbiome-derived indole-3-carboxaldehyde and prostaglandin D2 are effective promoters of rumen development

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Deciphering the biotic and abiotic drivers of coalescence asymmetry between soil and manure microbiomes

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New insights into functional divergence and adaptive evolution of uncultured bacteria in anammox community by complete genome-centric analysis

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9.7

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Feasibility of simultaneous optimization of Anammox start-up and nitrogen removal performance by intermittent dosing of nanoscale zero-valent iron

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9.7

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Alleviation of lipid metabolic dysfunction through regulation of intestinal bacteriophages and bacteria by green tea polyphenols in Ob/Ob mice

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8.5

华南农业大学

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Optimal balance of organic detritus and feed for fish growth and water quality improvement through regulating nutrients cycling

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中国科学院

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电 话:021-31021022

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上海凌恩生物科技有限公司(简称“凌恩生物”)成立于2014年,是一家从事组学科研技术服务的高新技术企业。凌恩生物拥有完备的组学测序技术平台,业务板块涉及组学研究的多个领域方向。截至2024年,凌恩生物已成立分公司或分支机构6家,服务范围辐射全球。

       以打造高品质测序及生物信息分析研发服务团队为目标,凌恩生物注重创新、时刻关注科研需求,先后研发了包括eDNA Metabarcoding,宏基因组元素循环分析,宏基因组抗性基因分析,高宿主污染去除宏基因组,宏转录组在内的微生态系列特色产品,备受广大科研工作者关注。凌恩技术团队参与的项目成果也多次发表在《Nature》《Cell》《PNAS》等国际高端学术期刊上秉持专业务实,追求卓越的理念,凌恩生物将满怀信心、迎接挑战,坚持应用新技术,研发新产品,助力科研。

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